产品简介

MCCOY'S 5A培养基含有25mM的HEPES。MCCOY'S 5A培养基渗透压：321±16 mOsm，MCCOY'S 5A培养基酸碱度：7.2－7.4。 MCCOY'S5A，withL-Glutamine，with25mMHEPES

MCCOY'S 5A

产品简介：1959, McCoy等报道了体外培养Novikoff Hepatoma细胞所需的氨基酸。他们在基本培养基5A的基础上通过调整加入氨基酸的量产生了MCCOY'S 5A的配方。随后Hsu 和Kellogg 使用这种培养基成功培养了一系列来源于多种组织的原代细胞，这些组织包括正常骨髓（bone marrow）、皮肤（skin）、牙龈（gingiva）、 睾丸（testes）、小鼠肾脏（mouse kidney）、网膜（omentum）、 肾上腺（adrenal glands）、肺（lung）、脾脏（spleen）、大鼠胚胎（rat embryos）及其它组织。

该产品含有25mM的HEPES。

产品类型：无菌过滤即用型液体培养基

渗 透 压：280-330 mOsm

酸 碱 度：7.2－7.4

参考文献：

1. McCoy, T.A., Maxwell, M. and Kruse, P.F., (1959). Amino Acid Requirement of the Novioff Hepatoma In Vitro. Proc. Soc. Exp. Biol. Med. 100, 115-118.

2. Patterson, M.K. and Dell’orco, R.T., (1978). Preparation of McCoy’s Medium 5A. Tissue Culture Association Manual. 4, 737-740.

3. Hsu, T.C. and Kellogg, D.S., (1960). Primary Cultivation and Continuous Propagation In Vitro of Tissues from Small Biopsy Specimens. J.N.C.I. 25, 221-231.

4. Iwakata, S. and Grace, J.T., (1964). Cultivation in Vitro of Myeoblasts from Human Leukemia. New York State Journal of Med. 64, 2279-2282.

5. Morton, H.J., (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89.

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注意：以上文献 仅供参考

MCCOY'S5A，withL-Glutamine，with25mMHEPES

MCCOY'S5A，withL-Glutamine，with25mMHEPES