产品简介

Human IFN-gamma索莱宝ELISA试剂盒的优势：
1，包被的酶标板单板可拆（能拆分成12个8孔的酶标条）
2，提供免费代测代检服务，代出实验数据
3，发文章高奖励
4，磁铁可吸附式包装盒，包装精美耐用

Human IFN-gamma 

Human IFN-gamma

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IFN-γ is added to detect the captured IFN-γ protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm