产品名称:anti-His Tag Mouse Monoclonal antibody(鼠单克隆抗体)
产品型号:M1001020
产品特点:anti-His Tag Mouse Monoclonal antibody(鼠单克隆抗体)在Western Blotting中使用内参其实就是在WB过程中的另外用内参对应的抗体检测内参,这样在检测目的产物的同时可以检测内参的表达,由于内参在各组织和细胞中的表达相对恒定,借助检测每个样品内参的量就可以用于校正上样误差,这样半定量的结果才更为可信。
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英文名称 anti-His Tag Mouse Monoclonal antibody
规 格 100ul
稀释比例 WB 1:3000 IP 1:200 IF 1:1000
浓 度 1mg/ml
保 存 Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment.
储存液 PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 61% Glycerol
性 状 Lyophilized or Liquid
克隆类型 Monoclonal Antibody
宿 主 Mouse
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
说明书
Anti-His Tag Mouse Monoclonal Antibody (3C5)
Catalog No. M1001020
Size 100μl
Storage: Store at -20°C. Do not aliquot the antibody.
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His)
residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags
can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers
identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Molecular Weight: N/A
Application: WB 1:1500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.
Specificity: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag
fusion proteins.
Form: Liquid ,1mg/ml
Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol
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RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody
RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody
Solarbio
Beijing Solarbio Science & Technology Co., Ltd
/82
Http://www.solarbio。。com
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