产品简介

anti-β-Actin Rabbit Polyclonal antibody（兔多克隆抗体）在Western Blotting中使用内参其实就是在WB过程中的另外用内参对应的抗体检测内参，这样在检测目的产物的同时可以检测内参的表达，由于内参在各组织和细胞中的表达相对恒定，借助检测每个样品内参的量就可以用于校正上样误差，这样半定量的结果才更为可信。

英文名称      anti-β-Actin Rabbit Polyclonal antibody 

别  名           Actin

应  用           WB

规  格           100ul

稀释比例      WB:1:1000

交叉反应      human,mouse

GeneID       60

蛋白分子量   42kDa

保  存           Store at -20°C. Avoid freeze / thaw cycles.Stable for one year from the date of shipment.

储存液          PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

纯化方法       Affinity purification

亚  型            unknown

免疫原          Synthetic Peptide

性  状           Lyophilized or Liquid

Swiss Prot  P60709

克隆类型      Polyclonal Antibody

宿  主           Rabbit

背景资料     Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

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