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产品名称:DMEM 高不含酚红谷氨酰胺丙酮酸钠 细胞培养

产品型号:90013

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产品特点:DMEM 高不含酚红谷氨酰胺丙酮酸钠 细胞培养
货号:90013
规格:500ml
别名:Dulbecco's Modified Eagle Medium
储存条件:2-8℃
单位:瓶

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90013DMEM 高不含酚红谷氨酰胺丙酮酸钠 细胞培养的详细资料:

DMEM 高不含酚红谷氨酰胺丙酮酸钠 细胞培养
货号:90013
规格:500ml
别名:Dulbecco's Modified Eagle Medium
储存条件:2-8℃
单位:

 DMEM 高不含酚红谷氨酰胺丙酮酸钠 细胞培养

DMEM是 dulbecco's modified eagle medium的缩写,是一种含各种氨基酸和葡萄糖的培养基,是在MEM培养基的基础上研制的。与MEM比较增加了各种成分用量,同时又分为高糖型(低于4500mg/L)和低糖型(低于1000mg/L)。高糖型有利于细胞停泊于一个位置生长,适于生长较快、附着较困难肿瘤细胞等。所以其特点主要包括以下:
(1)氨基酸含量为依格尔培养基的2倍,且含有非必需氨基酸,如甘氨酸等;
(2)维生素含量为依格尔培养基的4倍;
(3)含有糖酵解途径中的重要物质——丙酮酸;
(4)含有微量的铁离子.Eagle来自该培养基的发明人Harry Eagle

1640DMEM培养基的区别

1640培养基和DMEM培养基在组分和用途上有区别,主要是组分上的区别,查各自的配方表可知。用途上这两种培养基应用都很广泛,且都适合很多种细胞的培养。
    RPMI 1640主要用于悬浮细胞培养。可适用于哺乳动物、特殊造血细胞、正常或恶性增生的白细胞,杂交瘤细胞的培养。其它像K-562、HL-60、IM-9等成淋巴细胞、T细胞淋巴瘤细胞以及HCT-15上皮细胞等均可参考使用。
    DMEM适用于贴壁细胞生长,如各种实体瘤贴壁细胞。

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