产品简介

Anti-His Tag Monoclonal antibody抗体
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine （His） residues, often at the N- or C-terminus of the protein.

Anti-His Tag Monoclonal antibody抗体

英文名称 Anti-His Tag Monoclonal antibody

宿主 Mouse

别名 His Tag

应用 WB IF IP

稀释比例 WB 1:3000-8000. IP 1:2000-5000. IF 1:200-1000.

保存 Store at -20°C. Avoid freeze / thaw cycles.

储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.

纯化方法 Affinity purification

亚型 IgG1

免疫原 A synthetic peptide coupled to KLH

性状 液体

Public Immunogen Range A synthetic peptide coupled to KLH

克隆类型 Monoclonal Antibody

背景资料 A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

参考文献：

《Expression profiling and functional characterization of CD36 in turbot (Scophthalmus maximus L.)》 作者:ChaoLia1MengyuTiana1LuZhangaQiangFuaLinSongbNingYang 期刊:Fish & Shellfish Immunology 影响因子:3.298 PMID:30064021

《Expression profiling and microbial ligand binding analysis of high-mobility group box-1 (HMGB1) in turbot (Scophthalmus maximus?L.)》 作者:XinCaiaChengbinGaoaBaofengSubFenghuaTanaNingYangaGuodongWanga 期刊:Fish & Shellfish Immunology 影响因子:3.298 PMID:29679761

《The characterization and initial immune functional analysis of SCARA5 in turbot (Scophthalmus maximus?L.)》 作者:ChaoLia1MengyuTiana1LuZhangaQiangFuaLinSongbFeiChencNingYanga 期刊:Fish & Shellfish Immunology 影响因子:3.298 PMID:30006044

《Characterization, expression signatures and microbial binding analysis of cathepsin A in turbot, Scophthalmus maximus L.(SmCTSA)》 作者:Qiang Fua,Ning Yang,Chengbin Gao,Mengyu Tian,Shun Zhou,Xingjiang Mu,Fanyue Sun,ChaoLi 期刊:Fish & Shellfish Immunology 影响因子:3.298 PMID:29981472

《Identification and initial functional characterization of lysosomal integral membrane protein type 2 (LIMP-2) in turbot (Scophthalmus maximus L.)》 作者:Fenghua Tan,Min Cao,Xuefeng Ge,Chao Li,Mengyu Tian,Lu Zhang,Qiang Fu,Lin Song,Ning Yang 期刊:Developmental and Comparative Immunology 影响因子:3.119 PMID:31176756

Anti-His Tag Monoclonal antibody抗体